Figure 4

SDHB overexpression inhibited cell proliferation in SKOV3 cells. (A) SKOV3 cells were treated with pIRES2-SDHB or empty vector, then SDHB mRNA (24 h after treatment) and protein (48 h after treatment) were analysed by real time-PCR or western blot, respetively. (B) 48 h after transfection, cell viability of SDHB-overexpressed SKOV3 cells was examined using CCK8 assay for another 6 days. (C) Bcl-2 was downregulated after 48 h transfection in SKOV3 with β-actin as a loading control by western blot. (D) SKOV3 cells were treated with SDHB overexpression plasmid for 48 h, cell invasion and migration ability was evaluated as described previously. (E) MMP-2 and p-FAK, FAK were also analysed by western blot. (F) p-AMPKα, AMPKα were measured. Mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001.