Figure 5
From: Succinate dehydrogenase subunit B inhibits the AMPK-HIF-1α pathway in human ovarian cancer in vitro
SDHB modulated HIF-1α expression in ovarian cancer cells. SKOV3 and/ or A2780 cells were treated with SDHB siRNA (A) or over-expression plasmid (B) for 48 h, HIF-1α expression was analyzed by western blot with β-actin or β-tubulin as a loading control. (C) SKOV3 and A2780 cells were treated with CoCl2 at indicated concentrations for 24 h, HIF-1α and SDHB were examined by western blot.