WT1 suppresses granulosa cell apoptosis. (A) GCs transfected with WT1(−KTS) or empty vector were stained with TUNEL or WT1 antibody to visualize apoptotic cells (green) or WT1 protein (pink: merge with DAPI) and counterstained with DAPI to confirm nuclear status (blue). Representative fluorescence confocal microscopy images are shown. Magnification; X100 or X200. (B) Frequency of TUNEL-positive nuclei in GCs transfected with empty vector or WT1(−KTS). Data represent mean ± SD of five independent experiments. CT, empty vector-transfected cells; WT1, GCs transfected with WT1 (−KTS)(100 ng/well). * p < 0.05 vs. EV.