Figure 5
WT1 (−KTS) suppression of Bax promoter activity in immature rat GCs. (A) Cells were co-transfected with the Bax promoter luciferase reporter construct (250 ng/well) and either WT1 (+KTS) or (−KTS) (100 ng/well), and cultured for 24 h. FSH (50 ng/mL) was used as a positive control for Bax repressor. Cell lysates were assayed for the activity of the luciferase reporter gene. Luciferase activity is expressed as relative light units (RLU) and normalized based on β-gal activity in co-transfected cells. Values are calculated as fold-changes relative to control (CT), and are expressed as the mean ± SD of three independent experiments, each performed in triplicate. (B) Immunoblot analysis of WT1 protein in cultured GCs lysates to confirm WT1 overexpression. Bands corresponding to WT1 (52 kDa), and β-actin (42 kDa), respectively. CT, control (cells transfected with Bax promoter only). *p < 0.05 compared to control; **p < 0.05 compared to FSH.