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Figure 2 | Journal of Ovarian Research

Figure 2

From: Sequential combination therapy with flavopiridol and autocatalytic caspase-3 driven by amplified hTERT promoter synergistically suppresses human ovarian carcinoma growth in vitro and in mice

Figure 2

Western blot analysis of apoptosis-related proteins. The cells were plated in 100-mm plates and treated with AdHTVP2G5-rev-casp3/flavopiridol. Total protein extract of the cells was obtained, and Western blot analysis was performed as described in “Materials and methods.” Lane 1, untreated control; Lane 2, AdHTVP2G5-rev-casp3(MOI = 20) for 48 hs; Lane 3, 300 nM flavopiridol for 24 hs;Lane 4, AdHTVP2G5-rev-casp3(MOI = 20) for 48hs, followed by 300 nM flavopiridol. Western blot shown are representative of three independent experiments.

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Journal of Ovarian Research

ISSN: 1757-2215

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