Figure 4

In vivo antitumor activity. A: Subcutaneous tumors derived from OVCAR3 cells were treated as shown. Tumor volumes were monitored over time (days) after inoculation of tumor cells. Values represent the mean of 5 mice per group. B: 1:PBS, 2: flavopiridol (10 mg/kg), 3: AdHTVP2G5-rev-casp3 (7 × 10⁸ TCID50/mouse), 4: AdHTVP2G5-rev-casp3(7 × 10⁸ TCID50/mouse) + flavopiridol(5 mg/kg). Survival curves and mean survival durations for animals bearing abdominally spread OVCAR3 tumors. The animals received three treatments using PBS, flavopiridol, AdHTVP2G5-rev-casp3 or flavopiridol + AdHTVP2G5-rev-casp3 as described in the text. Survival was then monitored: the survival duration in animals receiving treatment using AdHTVP2G5-rev-casp3, flavopiridol or flavopiridol + AdHTVP2G5-rev-casp3 were significantly differently from those in the mice that received treatment using controls (P < 0.01). In addition, synergisms of AdHTVP2G5-rev-casp3 and flavopiridol were observed, and significantly prolonged survival can be achieved using combined AdHTVP2-G5-rev-casp3 and flavopiridol therapy without an increase in doses(P < 0.05).