Table 1 The apoptosis and cell cycle distributon of OVCAR3 cells after the sequential combination treatment of AdHTVP2G5-rev-casp3(MOI = 10)for 36 hrs or 72 hrs followed by flavopiridol (300 nM) for 48 hrs

Negative control

1.1 ± 2.9

51.7 ± 3.4

29.4 ± 5.2

18.9 ± 1.9

Flavopiridol(300nM) 24 hs

10.0 ± 3.2

63.8 ± 3.9

14.3 ± 1.5

21.9 ± 2.6

Ad. 36 hs

2.1 ± 0.3

52.6 ± 4.8

20.3 ± 2.7

27.1 ± 2.8

Ad.36 hs + DMSO 48 hs

2.5 ± 1.8

50.2 ± 3.8

21.9 ± 3.0

28.9 ± 3.1

Ad. 36 hs + Flavopiridol 48 hs

9.9 ± 4.6

48.5 ± 4.0

23.4 ± 3.1

28.1 ± 3.1

Ad.72 hs

4.8 ± 0.6

12.9 ± 2.1

67.8 ± 6.8*

19.3 ± 1.9

Ad.72 hs + DMSO 48 hs

5.2 ± 0.9

16.1 ± 2.6

62.5 ± 4.3

21.4 ± 2.2

Ad. 72 hs + Flavopiridol 48 hs

60.1 ± 5.3**

51.8 ± 3.8

22.3 ± 3.0

25.9 ± 3.1