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Table 2 Number and percentage of intact (Int) and degenerated (Deg) follicles of various stages evaluated in vitrified and non-vitrified ovarian tissue after Hematoxylin- eosin staining

From: Preservation of mouse ovarian tissue follicle morphology and ultra-structure after vitrifying in biotechnological protocols

Group

Total number of follicles

Number of primordial follicles (%)

Number of primary follicles (%)

Number of secondary follicles (%)

Total

Int

Deg

Stat

Total

Int

Deg

Stat

Total

Int

Deg

Stat

Control

329

156

154 (99)

2 (1)

-

103

101 (98)

2 (2)

-

70

67 (96)

3 (4)

-

CV1

308

142

136 (96)

6 (4)

-

99

90 (91)

9 (9)

a

67

38 (59)

29 (41)

a, b

CV2

310

148

142 (96)

6 (4)

-

88

81 (92)

7 (8)

-

74

48 (64)

26 (36)

a, b

CV3

299

140

134 (96)

6( 4)

-

90

81 (90)

9 (10)

-

69

42 (60)

27 (40)

a, b

CV4

309

141

135 (96)

6 (4)

-

94

85 (91)

9 (9)

a

74

48 (64)

26 (36)

a, b

CV5

338

165

159 (97)

6 (3)

-

97

93 (96)

4 (4)

-

76

59 (77)

17 (23)

a, b

CV6

316

152

147 (97)

5 (3)

-

98

94 (96)

4 (4)

-

66

52 (79)

14 (21)

a

CV7

337

162

158 (98)

3 (2)

-

104

101 (97)

3 (3)

-

71

67 (95)

4 (5)

-

  1. aPā€‰<ā€‰0.05 versus non-vitrified control group.
  2. bPā€‰<ā€‰0.05 versus vitrified tissue in CV7 group.
  3. CV, Conventional vitrification.