Metabolomics profiling of platinum sensitive A2780 and resistant C200 cell lines. (A) Partial least squares discriminant analysis (PLS-DA) score plot shows clear separation of metabolic profile of platinum sensitive A2780 and resistant C200. (B) Z-score plot of C200 metabolite intensities (RED, z-score range −18 to +174, truncated at 25) against A2780 metabolites (BLUE), taken as mean. Each dot represents 1 metabolite per observation. (C) Unsupervised hierarchical clustering of signature metabolites (N = 179) that separate A2780 vs C200 cells (t-test, p < 0.05). Yellow and blue indicate increased and decreased levels, respectively, with coloring for each metabolite relative to its mean observed intensity.