Figure 4

Conditioned media (CM) derived from irradiated organs are potent chemoattractants for A2780 cells. a. Chemotaxis of A2780 cells across Transwell membranes in response to CM harvested from BM, liver, and lung of irradiated and ibuprofen-treated SCID-beige inbred mice. b. Chemotaxis of A2780 cells across Transwell membranes in response to irradiated BM CM samples subjected to heat inactivation at different temperatures (left panel) and derived from molecular filtration by using Centricon centrifugal filtration devices (right panel). The chemotaxis assay was performed at least three times in duplicate, with similar results. Results are presented as mean ± SD, with a statistical significance *p < 0.05 or **p < 0.005 relative to the control (cells stimulated with RPMI 0.5% BSA medium).