Fig. 4
From: Novel reversible selective inhibitor of CRM1 for targeted therapy in ovarian cancer
S109 induces cell cycle arrest and nuclear retention of tumor suppressor proteins. a SKOV-3 cells were exposed to 2 μM of S109 for 24 h. Cells were harvested, stained with propidium iodide and analyzed by flow cytometry. b SKOV-3 cells were treated with S109 at the indicated concentrations for 24 h. Cells were then harvested and subjected to immunoblot analysis. c SKOV-3 cells were treated with S109 at the indicated concentrations for 24 h. Nuclear proteins was extracted and subjected to immunoblot analysis. d OVCAR-3 cells were treated with S109 at the indicated concentrations for 24 h. Nuclear proteins was extracted and subjected to immunoblot analysis