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Fig. 1 | Journal of Ovarian Research

Fig. 1

From: Lentivirus-mediated shRNA interference of clusterin blocks proliferation, motility, invasion and cell cycle in the ovarian cancer cells

Fig. 1

Establishment of stable CLU silencing OVCAR-3 and TOV-21G cell lines. a Detection of lentivirus transfection in OVCAR-3 and TOV-21G cells. The results of gene sequencing showed that OVCAR-3 and TOV-21G cells were transfected successfully LV-CLU or LV-NC gene with lentivirus (b) Analysis of levels of Clusterin protein in OVCAR-3 and TOV-21G cells. Results suggested that both OVCAR-3 and TOV-21G cells had the higher level of Clusterin expression. c Clusterin (GFP) expression in OVCAR-3 and TOV-21G cells as detected by fluorescence microscope (x100). The infection efficiency of OVCAR-3 cells (MOI 20) and TOV-21G cells (MOI 10) were shown. The cells were infected with LV-CLU or LV-NC. Four days later, the infection efficiency achieved was >90 %

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