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Fig. 1 | Journal of Ovarian Research

Fig. 1

From: Thymoquinone enhances cisplatin-response through direct tumor effects in a syngeneic mouse model of ovarian cancer

Fig. 1

TQ and cisplatin synergistically decrease cell viability in cultured ID8-NGL cells. a SRB assay showing effects of TQ and/or cisplatin (concentrations in μM, 72 h) in the mouse ovarian cancer cell line, ID8-NGL, expressed as a percentage of control. b Isobologram analysis for ID8-NGL cells showing Combination Index for Effective Doses (ED) ED50, ED75 and ED90. A Combination Index < 1 indicates a synergistic drug interaction between TQ and cisplatin. Values are mean ± SD for 3 experiments; *p < 0.01 below 1, Student’s t test. c Western blot analysis of cleaved PARP (CL PARP) following 24 h treatment of ID8-NGL cells with TQ (25 μM), cisplatin (1 μM) or the combination. Actin was used as a loading control. d Luciferase assay of ID8-NGL cells following 24 h treatment with vehicle, TQ (25 μM), cisplatin (1 μM) or the combination. Values are mean ± SD for 3 experiments; *p < 0.01 compared to control, Student’s t test

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