Fig. 1

Beclin-1 protein expression is maintained in high-grade serous ovarian tumors and ascites-derived cells. a Gene copy-number calls at the BECN1 locus are depicted for 569 ovarian serous cystadenocarcinoma tumors (teal & blue = heterozygous & homozygous deletion, respectively; red & pink = high-level & low-level amplification, respectively). OncoPrint generated using cBioPortal.org. b BECN1 protein expression data (RPPA) expressed as z-scores and GISTIC2-based copy-number calls for 398 samples were downloaded from cBioPortal and plotted. One-way ANOVA with Tukey’s Test was performed (NS: p > 0.05). Lines: Mean ± SEM. c Log₂-transformed and normalized RPPA data and GISTIC2-based copy-number estimates (log₂-transformed) for 398 samples were downloaded from UCSC’s Cancer Browser and plotted. d-f Beclin-1 expression was quantified in fresh-frozen samples of metastatic tumor tissue (d), primary cultures of ascites-derived cells (e), and early-passage ascites-derived cell lines (f). Quantification was performed using densitometry and depicted relative to Actin