Fig. 4

Ddx4 localization on immunomagnetic isolated OSC. Confocal microscopy showing a single OSC of small size with large nuclei counterstained by DAPI (C) and few cytoplasm. Ddx4, revealed by FITC (green) in B, localizes in vicinity of the membrane actin (red in A). By merging both FITC and PE, Ddx4 is localized outside of the nucleus and shows high fluorescence intensity. Other nuclei stained by DAPI belong to Ddx4-negative cells within the preparation