Fig. 3

Vaccination with CD117⁺CD44⁺CSC vaccine induced NK cell response against SKOV3 ovarian cancer. a FCM analysis results indicate the cytotoxicity of NK cells in mice immunized with the different vaccines, respectively. The ratio of the effector cells (splenocytes from the immunized mice) to the target cells (YAC-1cells) was 25:1; b A representative set of data for six mice were used to detect NK cytotoxicity; c and d The analysis results of qRT-PCR show the expression of perforin and granzyme B in ovarian cancer tissues, respectively. An experiment was repeated twice. Statistically significant differences are indicated by asterisk for *p < 0.05, **p < 0.01, and ***p < 0.003, respectively