Fig. 4

CD44⁺CD117⁺cell population in ovarian cancer tissues in nude mice. 2 × 10⁵ ovarian cancer tissue cell suspension in PBS were labeled with anti-Human/Mouse CD44 FITC 1:100, and anti-Human CD117 PE 1:20 antibodies for immunofluorescence detection. a FCM analysis shows that the number of CD44⁺CD117⁺double positive cells in CD117⁺CD44⁺CSC vaccine group was the lowest among 4 groups, whereas this number was the highest in PBS group; b Quantitative analysis of CD44⁺CD117⁺double positive cells in ovarian cancer tissues from the mice immunized with the different vaccines. *p <0.05, **p <0.01 and ***p <0.003; refer to the statistically significant differences as indicated