Table 2 List of enriched pathways (p <0.05), in which genes predicted to be targeted by differentially expressed miRNAs (P <0.05) between follicular fluid from hyperstimulated vs. unstimulated heifers

TGF-beta signaling pathway

miR-106b, −132, −148b-5p, −182, −212, −374a, −548j

1.75E-07

TGFBR2, TGFB2, SMAD2, SMAD3, SMAD4, BMPR2

Axon guidance

miR-22-5p, −30b, −31, −33a-3p, −182, −132, −550a

2.85E-06

EFNB1, DCC, EPHB4, EPHA3, PLXNA1, PAK4

MAPK signaling pathway

miR-30b, −106b, −132, −182, −212, −548j, −202-5p

6.43E-05

MAP3K1, MAP3K5, KRAS, MRAS, GRB2, FGF7

Endocytosis

miR-33a-3p, −106b, −182, −374a, −374b, −202-5p

3.83E-04

RAB11FIP4, RAB11FIP2, EEA1, IGF1R, EPS15, EPN

Colorectal cancer

miR-30b, −33a-3p, −106b, −132, −212, −384, −494

5.94E-04

SOS1, FZD3, SMAD2, DCC, MAPK1, BAX

Pathways in cancer

miR-33a-3p, −107, −132, −212, −494, −495, −548j

5.95E-04

E2F1, FGF18, WNT16, FGF7, PTEN, MITF

Wnt signaling pathway

miR-132, −212, −33a-3p, −494, −940, −495, −548j, −107

6.73E-04

LRP5, LRP6, TCF7, PLCB4, DVL3, WNT1, WNT5A

Neurotrophin signaling pathway

miR-106b, −30b, −940, −182, −212, −107,

0.001267

PIK3R2, NTRK2, NTRK3, RPS6KA6, IRS1, RAC1

Oocyte meiosis

miR-212, −132, −940, 495, −595, −107

0.003369

CDC27, CPEB1, PRKACB, FBXW11, MAPK1, RPS6KA3

GnRH signaling pathway

miR-940, −495

0.007085

PRKCA, SRC, MAP3K2, MAPK14, GRB2, MAP3K3

Pathways in cancer

miR-659, −141, −190, −449a, −200c, −361-5p, −145

9.51E-12

KRAS, PTEN, VEGFA, STAT5B, MITF, BCL2

MAPK signaling pathway

miR-23a, −23b, −141, −145, −200c, −92a, −125b, −24

2.94E-11

MKNK2, MEF2C, FGF, SOS1, RAS, MAerkPK2

Wnt signaling pathway

miR-125b, −449a, −302e, −145, −23a, −29b-2-5p, −659, −200c

2.76E-08

LRP5, LRP6, TCF7, PLCB4, DVL3, WNT1, WNT5A

ErbB signaling pathway

miR-23a, −23b, −125b, −206, −302e, −513c

3.19E-07

ERBB4, GRB2, SHC1, PIK3R3, PIK3CD, AKT3

Colorectal cancer

miR-659, 23a, −23b, −141, −190, −449a, −200c, −361-5p, −145

4.48E-07

DCC, APPL1, TGFBR2, SMAD3, SMAD4, APC

Axon guidance

miR-23a, −23b, −200c, −145

7.23E-07

PLXNC1, EFNB2, PAK4, DCC, SEMA6A, PAK7, MET

Neurotrophin signaling pathway

miR-302e, −361-5p, −452, −525-5p, −449a, −659

1.82E-06

NTRK2, NGFR, KRAS, MAP3K1, PIK3R3, PLCG1

Renal cell carcinoma

miR-659, −141, −190, −449a, −200c, −361-5p, −145

3.75E-05

GAB1, MET, SOS1, GRB2, VEGFA, NRAS

Focal adhesion

miR-24, −27b, −125a-3p, −139-5p, −206, −200c

5.04E-05

ITGB3, PXN, SHC3, ACTB, SRC, PAK2

Regulation of actin cytoskeleton

miR-200c, −145, −27b, −92a, −300, −206

8.29E-05

RAC1, ROCK2, GIT1, PIK3R3, ITGA3, LIMK1