Table 3 List of enriched pathways (P < 0.05), in which genes predicted to be targeted by differentially expressed miRNAs (P < 0.05) in blood plasma of hyperstimulated vs. unstimulated heifers

 Pathways in cancer

let-7 g, miR- 20b-3p, −22, −26b-3p, −34a, −221, −181c

1.44E-09

AKT3, PIK3R3, RASSF1, KRAS, MEK, DCC

 Wnt signaling pathway

let-7 g, miR-221, −32

4.04E-08

WNT1, SMAD2, DKK2, DVL3, VANGL2, CCND1

 Neurotrophin signaling pathway

let-7 g, miR-22, −181c, −221

6.73E-08

NTRK2, IRS2, AKT3, NGF, PIK3CD, NRAS

 Axon guidance

miR-34a, −181c, −221

2.19E-07

PLXNC1, DPYSL2, PAK1, MET, KRAS, EFNB1

 Endocytosis

miR-22, −34a, −130b-3p, −576-5p

1.23E-06

TGFBR1, RAB5A, RAB11FIP4, PDCD6IP, VPS37A

 MAPK signaling pathway

let-7 g, miR-22, −32, −34a, −221

2.69E-06

RRAS, MAP3K1, MAPK1, MAPK9, SRF, MAP2K4

 Colorectal cancer

let-7 g, miR-22, −26b-3p, −24-2-5p

4.59E-06

DCC, TGFBR1, KRAS, MAP2K1, AKT3, CASP3

 Chronic myeloid leukemia

miR-22, −181c, −221

4.98E-06

NRAS, AKT2, BCR, MAPK1, GAB2, BCL2L1

 TGF-beta signaling pathway

miR-26b-3p, −221, −22, −32

9.63E-06

ACVR2A, TGFBR2, SMAD2, SMAD4, BMP7

 ErbB signaling pathway

miR-22, −181c, −221

2.96E-05

ERBB4, GAB1, MAP2K1, PAK4, PIK3CD

 Pathways in cancer

miR-125b, −153, −410, −494

9.51E-12

DCC, GRB2, SMAD2, SOS1, E2F3

 MAPK signaling pathway

mir-125b, −153, −494

2.94E-11

MEF2C, MAPKAPK2, MAP3K1, TGFB2, FGFR2

 Wnt signaling pathway

miR-147, −153, −410, −494

2.76E-08

LRP6, WNT5A, DVL3, DKK2, PLCB1, ANGL2

 ErbB signaling pathway

miR-125b, −410

3.19E-07

ERBB4, SOS1, MAPK1, NRG3, GAB1, MAP2K7

 Colorectal cancer

miR-125b, −410, −153, −494

4.48E-07

DCC, BCL2, SMAD4, RAF1, SMAD2, PIK3R3

 Axon guidance

miR-147, −153, −410, −494

7.23E-07

PLXNA2, ROCK2, EFNA3, NFAT5, MAPK1

 Neurotrophin signaling pathway

miR-125b, −134, −147, −494

1.82E-06

NTF3, MAP3K1, SOS1, SORT1, BCL2

 Melanogenesis

miR-134, −99a-3p

4.52E-06

MAPK1, WNT5A, KRAS, GNAI3, CREB1

 Melanoma

miR-125b, −494

1.70E-05

E2F2, FGFR1, IGF1R, FGF7, RAF1

 Prostate cancer

miR-125b, −147, −153, −410, −494

1.82E-05

FGFR2,, IGF1R, MAPK1, IGF1, BCL2