Fig. 1
From: WAP four-disulfide core domain protein 2 gene(WFDC2) is a target of estrogen in ovarian cancer cells
E2 induces expression of WFDC2 in HO8910 cells. a Real-time RT-PCR analysis of the expression of WFDC2 and GAPDH in HO8910 cells after stimulation with E2 at different concentrations for 48 h (n = 3). The relative amounts of WFDC2 protein were determined using densitometry and normalized to GAPDH. *P <0.05. b Western blot analysis of the expression of WFDC2 and GAPDH in HO8910 cells after stimulation with E2 at different concentrations for 48 h (n = 3). The relative amounts of WFDC2 protein were determined using densitometry and normalized to GAPDH. *P < 0.05 versus the solvent control. c Western blot analysis of the expression of WFDC2 and GAPDH in HO8910 cells after stimulation with 625 ng/ml E2 at different time points. n = 3, *P < 0.05 versus 0 h. d Western blot analysis of the expression of WFDC2 and GAPDH in HO8910 cells incubated with TAM (100 ng/ml) for 8 h and then incubated with E2 (625 ng/ml) for 36 h. n = 3, *P < 0.05 versus the control