Fig. 2
From: WAP four-disulfide core domain protein 2 gene(WFDC2) is a target of estrogen in ovarian cancer cells
Knockdown of WFDC2 expression reduces cell proliferation and enables an E2 response in SK-OV-3 ovarian cancer cells. HO8910 (a) or SKOV-3 (b) cells were treated with or without E2 (625 ng/ml) and knockdown of WFDC2 reduced the viable cell number compared with the negative control. n = 3 *P < 0.05; **P < 0.01. E2 response of HO8910 (c) and SKOV3 (d) cells. Knockdown of WFDC2 enabled an E2 response in SK-OV-3 ovarian cancer cells. HO8910 (e) or SKOV-3 (f) cells were treated with E2 (625 ng/ml) alone or in combination with TAM (100 ng/ml), and knockdown of WFDC2 increased the inhibition efficiency in ovarian cancer cells by adding TAM compared with the control; *P < 0.05, **P < 0.01 for TAM versus the control. ∆P < 0.05, verus cells treated with TAM without ShRNA interference