Fig. 2
SERMs antagonize E2 in CD1 MOE. a, b qPCR analysis of (a) Pgr and (b) Greb1 induction in response to 48 h hormone starvation and 24 h treatment with DMSO, 1 nM E2 or 100 nM SERMs. “a” indicates significant difference compared to all treatments (p < 0.001) and “b” is significantly different than DMSO control treatment (p < 0.01). c Western blot analysis of CD1 MOE treated for 24 h with 1 nM E2 and 100 nM SERMs and the combination. d, e Densitometry of ERα and PRA bands relative to solvent treated cells normalized to actin. ERα is significantly lower in E2, RAL and DMA treated cells, while 4OHT does not result in ERα degradation. PR is significantly upregulated in E2 treated cells compared to all conditions. Significance determined by one-way ANOVA followed by Tukey’s post hoc test where * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001