Fig. 3
Analysis of primordial follicle morphology. a Illustration of primordial follicles considered morphologically normal (arrows) or abnormal (arrowheads). Abnormal primordial follicles were characterized by disorganized granulosa cells, shrunken ooplasm and/or pyknotic oocytes. The density of morphologically normal primordial follicles (log(number / mm2 + 1)) in the ovarian fragments under the different conditions, fresh (F), frozen-thawed (F-T), and after 2 (D2) and 6 days (D6) of culture, was quantified and compared between the control group and the b 10 μM S1P and c 10 μM Z-VAD-FMK groups. Scale bar: 250 μm. * p < 0.01. *** p < 0.0001