Fig. 2
From: Progesterone acts via the progesterone receptor to induce adamts proteases in ovarian cancer cells
Cell viability and ADAMTS mRNA expression in NIH-OVCAR-3 and ES-2 cell lines, after hormone treatment. a and b MTT viability assay of NIH-OVCAR-3 and ES-2 cell lines treated with 30 nM estrogen, 1 μM progesterone or 100 nM testosterone for 24 h compared to control. c and d ADAMTS 1 and 4 mRNA expression assessed by qPCR in the lysates of NIH-OVCAR-3 cells. GAPDH was used as normalizer. e and f ADAMTS 1 and 4 mRNA expression assessed by qPCR in the cell lysates of ES-2 cells. GAPDH was used as normalizer. Differences among groups were considered statistically significant when p ≤ 0.05. Experiments were performed in triplicate