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Fig. 3 | Journal of Ovarian Research

Fig. 3

From: Progesterone acts via the progesterone receptor to induce adamts proteases in ovarian cancer cells

Fig. 3

ADAMTS 1 and 4 protein expression of NIH-OVCAR-3 cell lysates and conditioned medium, after hormone treatment. a Representative Western blot of ADAMTS 1 and 4 in the lysates of NIH-OVCAR-3 cells treated with 30 nM estrogen (E), 1 μM progesterone (P), or 100 nM testosterone (T), compared to control (C). β-actin was used as loading control. b and c ADAMTS 1 and 4 protein expression in the lysates of NIH-OVCAR-3 cells. d Representative Western blot of ADAMTS 1 and 4 in the conditioned medium from NIH-OVCAR-2 cells. e and f ADAMTS 1 and 4 protein expression in the conditioned medium from NIH-OVCAR-3 cells. Conditioned medium equal sample loading was confirmed by Pounceau S staining (Additional file 1: Fig. S1a). Differences among groups were considered statistically significant when p ≤ 0.05. Experiments were performed in triplicate

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