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Fig. 4 | Journal of Ovarian Research

Fig. 4

From: Progesterone acts via the progesterone receptor to induce adamts proteases in ovarian cancer cells

Fig. 4

Progesterone induces increase of ADAMTS 1 and 4 in cell lysates and conditioned medium of ES-2 cell. a and c Representative Western blot of ADAMTS 1 and 4 in the lysates of ES-2 cells treated with 30 nM estrogen (E), 1 μM progesterone (P) or 100 nM testosterone (T), compared to control (C). β-actin was used as loading control. b and d ADAMTS 1 and 4 protein expression in the lysates of ES-2 cells. e Representative Western blot of ADAMTS 1 and 4 in the conditioned medium from ES-2 cells. f and g ADAMTS 1 and 4 protein expression in the conditioned medium from ES-2 cells. Conditioned medium equal sample loading was confirmed by Pounceau S staining (Additional file 1: Fig. S1b). Differences among groups were considered statistically significant when p ≤ 0.05. Experiments were performed in triplicate

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