Fig. 5
From: Progesterone acts via the progesterone receptor to induce adamts proteases in ovarian cancer cells
RU486 can rescue the increase of ADAMTS 1 induced by progesterone in NIH-OVCAR-3 conditioned medium. a and b Representative Western blot of ADAMTS 1 and 4 in the lysates of NIH-OVCAR-3 cells treated with progesterone (P) or progesterone and RU486 (P + RU486) compared to control (C). c and d ADAMTS 1 and 4 protein expression in the lysate of NIH-OVCAR-3 cells. e Representative Western blot of ADAMTS 1 and 4 in the conditioned medium from NIH-OVCAR-3 cells. f and g ADAMTS 1 and 4 protein expression in conditioned medium of NIH-OVCAR-3. Conditioned medium equal sample loading was confirmed by Pounceau S staining (Additional file 1: Fig. S1c). Differences among groups were considered statistically significant when p ≤ 0.05. Experiments were performed in triplicate