Fig. 6
From: Progesterone acts via the progesterone receptor to induce adamts proteases in ovarian cancer cells
RU486 can rescue the increase of ADAMTS 1 and 4 induced by progesterone in ES-2 cell lysates but not in the conditioned medium. a and b Representative Western blot of ADAMTS 1 and 4 in the lysates of ES-2 cells treated with progesterone (P) or progesterone and RU486 (P + RU486) compared to control (C). c and d ADAMTS 1 and 4 protein expression in the lysates of ES-2 cells. e Representative Western blot of ADAMTS 1 and 4 in the conditioned medium from ES-2 cells treated as above. f and g ADAMTS 1 and 4 protein expression in the conditioned medium from ES-2 cells. Conditioned medium equal sample loading was confirmed by Pounceau S staining (Additional file 1: Fig. S1d). Differences among groups were considered statistically significant when p ≤ 0.05. Experiments were performed in triplicate