Fig. 2

Photomicrographs of histologic sections from minipig ovaries. Higher expression of cleaved caspase-3 was detected in follicle cells in GV (bar = 50 μm; immunohistochemistry, 100×). a - animals that only underwent bilateral laparoscopic oophorectomy, the ovarian tissue of which was removed during oophorectomy for use as the control (GI); b - animals that underwent bilateral oophorectomy and immediate autologous transplantation of fresh ovarian tissue into the subcutaneous tissue (GII); c - animals that underwent bilateral oophorectomy and immediate autologous transplantation of fresh ovarian tissue into the peri-infundibular intraperitoneal site (GIII); d - animals that underwent bilateral oophorectomy and subsequent autologous transplantation of cryopreserved ovarian tissue into the subcutaneous tissue (GIV); e - animals that underwent bilateral oophorectomy and subsequent autologous transplantation of cryopreserved ovarian tissue into the peri-infundibular intraperitoneal ovarian region (GV)