Fig. 4From: A centrosome clustering protein, KIFC1, predicts aggressive disease course in serous ovarian adenocarcinomasHGSOC cell lines show higher incidence and severity of Centrosome amplification. a Confocal microscopic images showing the presence of centrosome amplification and clustering in ovarian cancer cell lines. Centrosomes and microtubules were visualized by immunostaining for γ-tubulin (green) and α-tubulin, respectively, and DNA was stained using DAPI (blue). Scale bar (white) 5 μm. b Bar graphical representation of percent cells showing centrosome amplification and multipolar mitosis in human ovarian cancer cell lines. 500 cells were counted in each case. c Immunoblots showing the levels of KIFC1 and centrosomal markers in ovarian cancer cells lines (KURAMOCHI, OVCAR3, OVSAHO, and SKOV3)Back to article page