Fig. 1

Expression of PRSS8 is specific to OVC cells. Expression of PRSS8 in normal and OVC cell lines (a), in tissue of normal ovary, OVC, and other types of cancer (b), and as detected in ISH (C – left) and IHC (C – right) in normal (N – in bottom left corner of ISH and IHC) and OVC tissues (magnification 20X and 40X). a Gene expression of PRSS8 in OVC cell lines was analyzed by qRT-PCR and normalized. Fold change represents the level of gene expression in OVC cell lines normalized against a normal ovarian cell line IOSE523. b Fold change represents the level of gene expression in cancer tissue samples normalized against the corresponding normal tissue; here, p < 0.001, PRSS8 expression in OVC vs. other cancer types; one-way ANOVA (SigmaStat). c (ISH) left, mRNA of tissues of ovarian tumors in normal individuals and OVC patients were hybridized In situ (20X magnification). (IHC) right, Detection of prostasin levels by immunohistochemistry. Nuclear stain is indicated with solid arrows