Fig. 2From: Overexpression of Oct4 in porcine ovarian stem/stromal cells enhances differentiation of oocyte-like cells in vitro and ovarian follicular formation in vivoExpression of pluripotency-related transcription factors in OvSCs and Oct4-OvSCs analyzed by RT-PCR and western blot. a Result of RT-PCR for exogenous Oct4, endogenous Oct4, Sox2 and Nanog. b Western blot analysis for Oct3/4, Sox2 and Nanog. β-actin was used as a reference. c Relative mRNA abundance of endogenous Oct4, Sox2 and Nanog with a base of b-actin intensity. d Relative protein abundance of Oct3/4, Sox2 and Nanog with a base of β-actin intensity. Three independent experiments performed in triplicates were used for C and D (*p < 0.05)Back to article page