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Fig. 3 | Journal of Ovarian Research

Fig. 3

From: Overexpression of Oct4 in porcine ovarian stem/stromal cells enhances differentiation of oocyte-like cells in vitro and ovarian follicular formation in vivo

Fig. 3

In vitro differentiation of OvSCs and Oct4-OvSCs into oocyte-like cells. a and b Following the induction, OvSCs (a a and a b) and Oct4-OvSCs (b a and b b) were changed to distinct sphere-like morphology during the period of 15 to 30 days of induction. At day 45 of differentiation, the cells were changed their morphology to ‘big round-cells’, so called OLCs in OvSCs (a c-a e) and Oct4-OvSCs (b c-b e). Zona pellucida (Black arrow) was observed in OLCs (a d and b d). Nuclear staining by DAPI was observed in OLCs differentiated from OvSCs (a e) and Oct4-OvSCs (b e). OLCs derived from Oct4-OvSCs expressed red fluorescence protein through cytoplasm (b e). Scale bar = 50 μm. c The number and diameter of OLCs from OvSCs and Oct4-OvSCs. At day 45 of differentiation, the floating cells were counted and measured the diameter of the cells (Three independent experiments performed in 8 replicates, *p < 0.05)

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