Fig. 4

The expression of transcriptional, germ cell specific and folliculogenesis markers following the differentiation into OLCs. a These markers were analyzed on day 0, 15, 30 and 45 of differentiation. β-actin was used as a reference gene. Porcine ovarian tissue was used for positive control. b Relative mRNA abundance of Oct4, Vasa, GDF9b, C-Mos, FSHR, C-kit, Stella and ZPC with a base of β-actin intensity. Different superscript letters represent a significant difference between OvSCs and Oct4-OvSC on day 0 or 45 of differentiation. Asterisk indicates a significant difference between the day 0 and 45 of differentiation in OvSCs or Oct4-OvSCs. Three independent experiments performed in triplicates were used and multiple comparisons were performed by Duncan test (Mean ± S.E, p < 0.05)