Skip to main content
Fig. 6 | Journal of Ovarian Research

Fig. 6

From: Overexpression of Oct4 in porcine ovarian stem/stromal cells enhances differentiation of oocyte-like cells in vitro and ovarian follicular formation in vivo

Fig. 6

Histological observation of mouse ovaries following cell transplantation into infertile mouse. a Histological observation by H&E staining. Image of normal folliculogenesis in BALB/C nude mice ovary (a a). After twice busulphan injection at 2 weeks interval, follicles and germ cells were destroyed completely in ovaries when observed at 2 and 6 weeks after the second injection (a b and a c, respectively). Cells or PBS injection was performed at 2 weeks after the second busulphan injection. Injection of AFs or PBS into sterile ovaries (a d and a e, respectively). Transplantation of OvSCs (a f and a g) and Oct4-OvSCs (a h-a j). White arrows indicate new primary follicles while black arrows indicate a developing follicle containing theca and groanulosa cells. b Histological observation by DAPI staining. Image of normal folliculogenesis (b a). Sterile mouse ovary at 6 weeks after the second busulphan injection (b b). Ovaries injected AFs or PBS (b c and b d, respectively). Transplantation of OvSCs (b e) and Oct4-OvSCs (b f-b h). Red fluorescence indicates PKH26 labeled cells and blue florescence indicates nucleus. Scale bar = 100 μm

Back to article page