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Table 1 RT-PCR primers specific to porcine MSCs and differentiated cells

From: Overexpression of Oct4 in porcine ovarian stem/stromal cells enhances differentiation of oocyte-like cells in vitro and ovarian follicular formation in vivo

Gene Primer sequence (5′-3′) Product Size (bp) Annealing Tm (°C) Reference/Accession number
OCT4 F-AGGTGTTCAGCCAAACGACC 335 60 Carlin et al. [12]
R-TGATCGTTTGCCCTTCTGGC
SOX2 F-GCCTGGGCGCCGAGTGGA 443 64 Carlin et al. [12]
R-GGGCGAGCCGTTCATGTAGGTCTG
Nanog F-ATCCAGCTTGTCCCCAAAG 438 60 Carlin et al. [12]
R-ATTTCATTCGCTGGTTCTGG
GDF9b F-GGATCCAGAAAAGCACAACC 227 56 Dyce et al. [5]
R-AGTGTCCAGGGATGAAATGC
FSHR F-CTCACCTACCCCAGCCACT 243 56 Dyce et al. [5]
R-CTCAGGGGAGCAAGTCACAT
C-MOS F-AAATCAGCGACTTTGGTTGC 200 56 Dyce et al. [5]
R-CTGACGCTCCCCTGAGTAAG
VASA F-TTGCAGGACGAGATTTGATG 165 56 Dyce et al. [5]
R-CCAATTCTCGAGTTGGTGC
ZPC F-TGGTGTACAGCACCTTCCTG 202 56 Dyce et al. [5]
R-ATCAGGCGCAGAGAGAACAC
Stella F-TTAATCCAACCCGGACTCAG 173 56 Linher et al. [7]
R-TGGTTGAGGTGGATATTCTGG
C-kit F-TGTATTCACAGAGACTTGGCGG 124 56 Linher et al. [7]
R-CGTTTCCTTTGACCACGTAA
Β-Actin F-CTCGATCATGAAGTGCGACG 114 58 SSU07786
R-GTGATCTCCTTCTGCATCCTGTC