Fig. 4

1,25-dihydroxyvitamin D3 suppresses proliferation of human and murine germline-derived cell lines. Proliferation of the A2780 human ovarian cancer cell line (Panel a) as well as the NTERA-2 human teratocarcinoma (Panel b) and mP19 murine embryonal teratocarcinoma (Panel c) cell lines was significantly decreased by 1,25-dihydroxyvitamin D3 in a dose-dependent manner in comparison with cells treated with vehicle only. All proliferation experiments were done either in RPMI (for A2780), DMEM (for NTERA-2), or αMEM (for P19) culture medium containing 0.5 % BSA for 72 h using 0.3 × 10⁴ cells/well in a 96-well plate. The negative control values are normalized to 100 %. For each cell line, the experiment was repeated twice in triplicate with similar results