Fig. 5

1,25-dihydroxyvitamin D3 inhibits metastasis of human ovarian cancer and teratocarcinoma cells. Detection of in vivo-transplanted 1,25-dihydroxyvitamin D3-treated A2780 human ovarian cancer cells (a) and hNTERA-2 human teratocarcinoma cells (b) in the organs of irradiated mice post transplantation. As shown, the number of 1,25-dihydroxyvitamin D3-treated cancer cells was significantly lower in isolated organs from mice than in ex vivo-untreated cells (vehicle only). Detection was performed by employing RT-qPCR for the presence of human Alu sequences in purified genomic DNA samples. For statistical comparisons, a one-way analysis of variance and a Tukey’s test for post hoc analysis were carried out, and means ± SD are shown. Significance levels: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 versus control (untreated) cells