Fig. 2

CRISPR/Cas-mediated HE4 downregulation reduces chemoresistance in SKOV3-C1 cells. a SKOV3-C1 cells were transfected with a human CRISPR-HE4 Double Nickase plasmid and CRISPR-Control Double Nickase plasmid for 48 h. Transfected cells were selected with puromycin and efficient downregulation of HE4 was measured by qRT-PCR and ELISA. [HE4] = concentration of HE4 in supernatant as measured by ELISA. b-c CRISPR-Control and CRISPR-HE4 cells were treated with DMSO vehicle or 25 μM cisplatin or 5 nM paclitaxel for 24 h, and MTS assay was performed to measure cell viability. Results are displayed as percent survival of vehicle treated cells. Error bars represent the standard deviation of three or more biological replicates, *p < .05