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Fig. 4 | Journal of Ovarian Research

Fig. 4

From: HE4 promotes collateral resistance to cisplatin and paclitaxel in ovarian cancer cells

Fig. 4

EGR1 mRNA and protein upregulation is suppressed in HE4-overexpressing clones. a Quantitative RT-PCR validation of microarray using RNA from SKOV3-NV, SKOV3-C1, and SKOV3-C7 cells treated with vehicle (DMSO) or 25 μM cisplatin. Error bars represent standard deviation of three biological replicates, *p < .05, **p < .005. b Timecourse analysis of EGR1 expression following treatment of SKOV3-NV and SKOV3-C1 cells with 25 μM cisplatin. c EGR1, phosphorylated ERK, and total ERK protein levels following treatment with vehicle (DMSO), 25 μM cisplatin, or 10 ng/mL rEGF (positive control) were determined by western blot. GAPDH was used as a loading control. d-e Densitometry analysis of EGR1 (normalized to GAPDH) and phospho-ERK/ERK ratio, from western blot in (c)

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