Table 3 Genes Differentially Expressed Upon Cisplatin Treatment of SKOV3-NV and SKOV3-C1 Cells. SKOV3-NV and SKOV3-C1 cells were treated with vehicle (DMSO) or 25 μM cisplatin for 24 h (n = 3/group). RNA was isolated and Affymetrix HTA 2.0 arrays were performed to determine cisplatin-induced differences in gene expression in SKOV3-NV and SKOV3-C1 cells. The top fifteen annotated, protein-coding genes (p < 0.05) differentially expressed in either direction are listed below, as well as EGR1, which was the sixteenth gene change for SKOV3-NV cells
From: HE4 promotes collateral resistance to cisplatin and paclitaxel in ovarian cancer cells
SKOV3-NV-veh vs. SKOV3-NV-cis | SKOV3-C1-veh vs. SKOV3-C1-cis | ||||||
|---|---|---|---|---|---|---|---|
Gene symbol | Description | Fold-change | ANOVA p-value | Gene symbol | Description | Fold-change | ANOVA p-value |
PRKCA | Protein kinase C, alpha | 7.21 | 0.000002 | PRKCA | Protein kinase C, alpha | 5.39 | 0.002139 |
SBF2 | SET binding factor 2 | 6.27 | 4.50E-07 | TMEM117 | Transmembrane protein 117 | 5.24 | 0.001217 |
CDH6 | Cadherin 6, type 2, K-cadherin (fetal kidney) | 5.36 | 0.000009 | PTPRG | Protein tyrosine phosphatase, receptor type, G | 5.22 | 0.000633 |
DYM | Dymeclin | 5.24 | 2.43E-07 | PTPRM | Protein tyrosine phosphatase, receptor type, M | 4.89 | 0.000015 |
DPYD | Dihydropyrimidine dehydrogenase | 5.17 | 0.000011 | MSI2 | Musashi RNA-binding protein 2 | 4.79 | 0.013201 |
MSI2 | Musashi RNA-binding protein 2 | 5.04 | 0.00001 | CDKAL1 | CDK5 regulatory subunit associated protein 1-like 1 | 4.69 | 0.005495 |
PTPRM | Protein tyrosine phosphatase, receptor type, M | 4.99 | 0.000004 | DPYD | Dihydropyrimidine dehydrogenase | 4.61 | 0.001272 |
EXOC6B | Exocyst complex component 6B | 4.95 | 0.000001 | PITPNC1 | Phosphatidylinositol transfer protein, cytoplasmic 1 | 4.56 | 0.000222 |
CDKAL1 | CDK5 regulatory subunit associated protein 1-like 1 | 4.9 | 0.000013 | SBF2 | SET binding factor 2 | 4.29 | 0.000294 |
TMEM117 | Transmembrane protein 117 | 4.81 | 0.000003 | SAMD12 | Sterile alpha motif domain containing 12 | 4.19 | 0.004401 |
MAML2 | Mastermind-like 2 (Drosophila) | 4.78 | 0.000041 | TENM1 | Teneurin transmembrane protein 1 | 4.13 | 0.000457 |
SPTLC3 | Serine palmitoyltransferase, long chain base subunit 3 | 4.76 | 0.000016 | GTDC1 | Glycosyltransferase-like domain containing 1 | 3.99 | 0.007196 |
PTPRG | Protein tyrosine phosphatase, receptor type, G | 4.67 | 9.73E-07 | EXOC6B | Exocyst complex component 6B | 3.98 | 0.002653 |
TRHDE | Thyrotropin-releasing hormone degrading enzyme | 4.66 | 0.000011 | CDH13 | Cadherin 13, H-cadherin (heart) | 3.83 | 0.000243 |
FTO | Fat mass and obesity associated | 4.57 | 2.54E-07 | FTO | Fat mass and obesity associated | 3.69 | 0.009 |
EGR1 | Early growth response 1 | -3.51 | 0.000319 | -- | -- | -- | -- |
FRG2 | FSHD region gene 2; FSHD region gene 2-like | -3.57 | 0.000156 | SNAI1 | (snail family zinc finger 1) | -2.16 | .000605 |
PPP1R15A | Protein phosphatase 1, regulatory subunit 15A | -3.69 | 0.000047 | NR4A3 | Nuclear receptor subfamily 4, group A, member 3 | -2.17 | 0.00205 |
SNAI1 | Snail family zinc finger 1 | -3.85 | 0.00028 | PMAIP1 | Phorbol-12-myristate-13-acetate-induced protein 1 | -2.22 | 2.51E-07 |
HBEGF | Heparin-binding EGF-like growth factor | -4.01 | 0.000148 | FRG2 | FSHD region gene 2; FSHD region gene 2-like | -2.24 | 0.033969 |
NR4A3 | Nuclear receptor subfamily 4, group A, member 3 | -4.05 | 0.000145 | GADD45A | Growth arrest and DNA-damage-inducible, alpha | -2.26 | 0.000042 |
TNFAIP3 | Tumor necrosis factor, alpha-induced protein 3 | -4.23 | 0.000031 | CCL26 | Chemokine (C-C motif) ligand 26 | -2.37 | 0.023835 |
GADD45A | Growth arrest and DNA-damage-inducible, alpha | -4.23 | 0.000007 | RND1 | Rho family GTPase 1 | -2.58 | 0.011645 |
DDIT3 | DNA-damage-inducible transcript 3 | -4.26 | 0.000017 | TNFAIP3 | Tumor necrosis factor, alpha-induced protein 3 | -2.61 | 0.001029 |
ARL14 | ADP-ribosylation factor-like 14 | -4.28 | 0.000109 | CCL20 | Chemokine (C-C motif) ligand 20 | -2.64 | 0.000022 |
TNF | Tumor necrosis factor | -4.38 | 0.000048 | BIRC3 | Baculoviral IAP repeat containing 3 | -2.71 | 0.000066 |
CCL20 | Chemokine (C-C motif) ligand 20 | -4.57 | 0.000051 | HBEGF | Heparin-binding EGF-like growth factor | -2.96 | 0.000133 |
RND1 | Rho family GTPase 1 | -4.61 | 0.000069 | ATF3 | Activating transcription factor 3 | -2.96 | 0.000575 |
ATF3 | Activating transcription factor 3 | -6.24 | 0.000007 | IL1A | Interleukin 1, alpha | -3.36 | 0.00006 |
ZNF280A | Zinc finger protein 280A | -6.28 | 0.000082 | ZNF280A | Zinc finger protein 280A | -3.78 | 0.000008 |
IL8 | Interleukin 8 | -10.71 | 0.000041 | IL8 | Interleukin 8 | -6.41 | 0.000002 |