Fig. 2

Validation of CRAd-mediated expression of IL-24 and ING4 genes. a) The concentrations of IL-24 protein following infection of the indicated OvCa cell lines and normal IOSE523 cells with CRAd-IL24 at the MOIs of 1 and 10 vp/cell were determined in cell culture supernatants 3 days postinfection using commercial ELISA kit with IL-24 concentration standards. Each bar represents the cumulative mean ± SD (*p ≤ 0.05). b) The relative levels of ING4 gene expression were determined following infection of SKOV3ip.1 cells with CRAd-ING4 at the MOIs of 100, 33, and 11 vp/cell. The ING4 protein band of 29 kDa was detected in cell lysates 3 days postinfection with 100, 33, and 11 vp/cell, but not in mock-infected cells (0 vp/cell) using Western blot with rabbit polyclonal Ab raised against ING4 internal region