Fig. 6
Evaluation of oncolytic effects of CRAd-IL24 or CRAd-ING4 alone and combined together. Monolayers of SKOV3ip.1, SKOV3luc, OVCAR3, and OV-4 cells were infected with each CRAd alone (left panel) or two CRAd vectors together (right panel) at the indicated MOIs. Oncolytic effects of CRAd vectors were assessed by determining cell monolayer integrity 6 days postinfection. The integrity of cell monolayers was determined by staining adherent cells with crystal violet. The stained monolayers were scanned using a plate reader set at 565 nm to calculate the percentage cell density in monolayers infected with each viral dose that were determined with respect to the uninfected control. Each data point represents the cumulative mean ± SD (error bars are smaller than the symbols, p ≤ 0.05)