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Fig. 7 | Journal of Ovarian Research

Fig. 7

From: Combinatorial strategies based on CRAd-IL24 and CRAd-ING4 virotherapy with anti-angiogenesis treatment for ovarian cancer

Fig. 7

Monitoring cytotoxic effects induced by CRAd-IL24 or CRAd-ING4 alone and together. The monolayers of SKOV3ip.1, OvCa OV-4, or OVCAR3 ovarian cancer cells and normal ovarian surface epithelial IOSE-120 cells were infected with each CRAd alone (left panel) or two CRAd vectors together (right panel) at MOI of 1 vp/cell. The infected and uninfected cells were subjected to CellTox™ Green Cytotoxicity assay (Promega) by adding DNA-binding cyanine dye on day 3 and monitoring the increase in fluorescent signal intensity proportional to cytotoxicity till day 5 postinfection to detect the level of CRAd-mediated cell killing using the Synergy-HT plate reader with 485 nm excitation and 520 nm emission wavelength filters. Each data point represents the cumulative mean ± SD (some error bars are smaller than the symbols, p ≤ 0.05)

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