Fig. 1

Ovarian bioreactor components and schematic of bioreactor setup. a Ovaries were first cannulated and tied off using suture. Ovaries were then placed in a customized bioreactor consisting of (b) a modified GLS 80 cap fitted with inlet and outlet ports for media flow and (c) a polypropylene ovary holder. d All bioreactor components were housed in a 1000 mL bottle with short rods connecting the ovary holder to the cap. e The entire bioreactor and media reservoir bottle were placed in an incubator to maintain an in vivo environment while the waste media bottle was placed outside the incubator. A digital multi-channel pump controlled and maintained media flow into the ovary via the cannula