Fig. 2

Nutrient distribution across bovine ovaries. Stained water was run through the ovarian vasculature using our cannulation method at 1.5 mL/min, and ovaries were dissected (a) immediately following harvest, (b) 10 min of fluid flow testing, and (c) 60 min of fluid flow testing. After extended flow, the ovarian vasculature and tissue became stained with trypan, demonstrating fluid was successfully administered via the native vasculature. c Over short-term culture, whole ovary mass was observed to quickly increase, but began to slow at approximately 15 min, indicating saturation (Logarithmic trendline, R ² = 0.982), e Over short-term testing, a small increase in outflow rate was observed as the ovary rapidly absorbed fluid. Outflow rate subsequently began to level off as the tissue became saturated with fluid (Logarithmic trendline, R ² = 0.885). Data presented as mean ± SE (n = 2)