An ovarian bioreactor for in vitro culture of the whole bovine ovary: a preliminary report

Table 1 Flow rate and pH measurements of the media

	Culture condition	Mean ± SEM
Average Media Flow Rate (mL/min)	Fresh media circulation (Group 1)	0.856 ± 0.023
Average Media Flow Rate (mL/min)	DXR treatment in fresh media (Group 3)	0.880 ± 0.046
Average Media pH	Fresh media circulation (Group 1)	7.72 ± 0.04
	Recirculated media (Group 2)	*6.27 ± 0.51
	DXR treatment in fresh media (Group 3)	7.76 ± 0.14

Flow rates into the ovary were calculated from fresh media circulation (Group 1) and 500 nM DXR treatments (Group 3) conditions. Flow rates are reported as bulk averages taken over 6, 12 and 24 h time points to calculate the mean and SEM at all time points. PH was measured from ovaries perfused with fresh media (Group 1), recirculation of used media (Group 2), and DXR treatment of fresh media (Group 3). Media was removed from the waste reservoir at 12, 24, and 48 h time points to measure pH and determine the effect of the different treatment groups on the bioreactor culture environment. Data presented as the mean and SE across all time points
*p < 0.05, two-way ANOVA

ISSN: 1757-2215