Fig. 2
Expression patterns of circadian genes after testosterone treatment in human luteinized granulosa cells. Human luteinized granulosa cells were exposed to 100 ng/mL testosterone dissolved in serum-free medium for 2 h and cells in the control group were cultured in serum-free medium without treatment. Samples were harvested every 4 h from the beginning of treatment for 48 h. Each value represents the mean ± SEM of three independent experiments. Significant statistical differences are shown as below: the testosterone group PER2 P4 vs. 24 = 0.028, P24 vs. 32 = 0.041, P24 vs. 48 = 0.039, P 4 vs. 44 = 0.024, CLOCK P4 vs. 24 = 0.04; the control group PER2 P4 vs. 16 = 0.016, CLOCK P4 vs. 12 = 0.022, P4 vs. 16 = 0.031, P4 vs. 20 = 0.006, P4 vs. 36 = 0.011