Fig. 2

Effect of in vitro HDAC inhibition on acetylated histone and tubulin. OVCAR8 and SKOV3 cells were treated with 2 μM SAHA, 7.5 nM LBH589 or vehicle for 16 h. Total cellular protein was isolated and subjected to Western blot analysis to determine the relative levels of acetylated tubulin, acetylated histone H3 and Aurora-A kinase. β-actin was used as a protein loading control