Fig. 3

Representative images of immunohistochemical analysis of Bcl2, Ki67 and HSP70 levels in cortical human ovarian tissue. a microphotography shows Bcl2 staining in fresh stromal cells, compared to cryopreservation tissue in PP (b) and quartz (c). For follicles a positive Bcl2 staining was found in GCs after cryopreservation in PP (d) and quartz (e). Vessels evaluation shows a positive staining for Bcl2 both in fresh tissue (f) and cryopreserved tissue using PP (h) or quartz vial (g). For ki67 a positive staining after cryopreservation is shown in the nuclei like in the ooplasm using PP vial (i) and quartz vial (l). For hsp70 a diffuse positive staining in stromal cells is shows in fresh (m) and cryopreserved tissue using PP (n) or quartz devices (o). Cells stained positive for Bcl2, Ki67, HSP70 are brown, compared with the blue counterstained cells. Magnification from 25× to 400×